Search results for " culture"
showing 10 items of 5779 documents
Design and experimental validation of a magnetic device for stem cell culture.
2020
Cell culture of bone and tendon tissues requires mechanical stimulation of the cells in order to mimic their physiological state. In the present work, a device has been conceived and developed to generate a controlled magnetic field with a homogeneous gradient in the working space. The design requirement was to maximize the magnetic flux gradient, assuring a minimum magnetizing value in a 15 mm × 15 mm working area, which highly increases the normal operating range of this sort of devices. The objective is to use the machine for two types of biological tests: magnetic irradiation of biological samples and force generation on paramagnetic particles embedded in scaffolds for cell culture. The…
Synthesis and in vitro biological evaluation of novel diaminothiophene scaffolds as antitumor and anti-influenza virus agents. Part 2
2017
On the basis of high-throughput screening, fragment-based drug discovery, structure–activity relationships and building block analysis methods, herein we report the synthesis and biological evaluation of a novel series of diethyl 2,5-diaminothiophene-3,4-dicarboxylate derivatives. All of the prepared Schiff bases (with mono-, di- and poly-substituents at the aromatic portion), mono- and bis-amides of diethyl 2,5-diaminothiophene-3,4-dicarboxylate, were evaluated against various human cancer and non-cancerous (only for active compounds) cell lines, as well as influenza A (subtypes FM/1/47/H1N1, hanfang/359/95/H3N2) and B (subtype jifang/13/97) viruses. The obtained results suggest that some …
A multisensor fusion approach to improve LAI time series
2011
International audience; High-quality and gap-free satellite time series are required for reliable terrestrial monitoring. Moderate resolution sensors provide continuous observations at global scale for monitoring spatial and temporal variations of land surface characteristics. However, the full potential of remote sensing systems is often hampered by poor quality or missing data caused by clouds, aerosols, snow cover, algorithms and instrumentation problems. A multisensor fusion approach is here proposed to improve the spatio-temporal continuity, consistency and accuracy of current satellite products. It is based on the use of neural networks, gap filling and temporal smoothing techniques. …
Land set-up systems in Italy: A long tradition of soil and water conservation sewed up to a variety of pedo-climatic environments
2020
Land set-up systems in Italy were and, in a few cases, still are integral parts of agricultural landscapes. The soils of Italy mirror a wide variety of climates and morphologies and derive from a great diversity of parent materials influenced by different soil temperature and moisture regimes. Furthermore, their development was influenced by several anthropogenic activities over time including land settings. Land set-up systems have been widely used throughout the Italian soilscape to regulate surface and underground water and to improve the physical soil features in a way that ensures mixed herbaceous and tree-crops cultivation. In recent times, new technologies have been introduced in agr…
Responses of aquatic plants to eutrophication in rivers:a revised conceptual model
2018
Compared to research on eutrophication in lakes, there has been significantly less work carried out on rivers despite the importance of the topic. However, over the last decade, there has been a surge of interest in the response of aquatic plants to eutrophication in rivers. This is an area of applied research and the work has been driven by the widespread nature of the impacts and the significant opportunities for system remediation. A conceptual model has been put forward to describe how aquatic plants respond to eutrophication. Since the model was created, there have been substantial increases in our understanding of a number of the underlying processes. For example, we now know the thre…
Cultural evolution and environmental change in Central Europe between 40 and 15 ka
2020
The role of environmental change in the evolution of cultural traits is a topic of long-standing scientific debate with strongly contrasting views. Major obstacles for assessing environmental impacts on the evolution of material culture are the fragmentary nature of archaeological and – to a somewhat lesser extent – geoscientific archives and the insufficient chronological resolution of these archives and related proxy data. Together these aspects are causing difficulties in data synchronization. By no means does this paper attempt to solve these issues, but rather aims at shifting the focus from demonstrating strict chains of causes and events to describing roughly contemporaneous developm…
Iberian Neolithic Networks: The Rise and Fall of the Cardial World
2017
Recent approaches have described the evolutionary dynamics of the first Neolithic societies as a cycle of rise and fall. Several authors, using mainly c14 dates as a demographic proxy, identified a general pattern of a boom in population coincident with the arrival of food production economies followed by a rapid decline some centuries afterwards in multiple European regions. Concerning Iberia, we also noted that this phenomenon correlates with an initial development of archaeological entities (i.e., ‘cultures’) over large areas (e.g. the Impresso-Cardial in West Mediterranean), followed by a phase of ‘cultural fragmentation’ by the end of Early Neolithic. These results in a picture of high…
Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defe…
2020
Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic…
Highly efficient construction of infectious viroid-derived clones
2019
[Background] Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriented reaction requiring tedious screenings and (iii) additional steps are required for cloning into a binary vector for agroinfiltration or for in vitro RNA production.
Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes
2015
Abstract: In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the geno…